Journal: Disease Markers

Article Title: Upregulation of SERPINE2 Results in Poor Prognosis of Hepatoblastoma via Promoting Invasion Abilities

doi: 10.1155/2022/2283541

Figure Lengend Snippet: Bioinformatics analysis and SERPINE2 expression in hepatoblastoma. (a) The cluster dendrogram of genes. Genes that could not be clustered into one of these modules were assigned to the gray module. Every gene represents a line in the hierarchical cluster. (b) Heatmap of the module-trait relationships. (c) Scatterplots of gene significance (GS) versus module significance (MS) in brown modules. (d) PPI networks show the interaction of genes related to HB metastasis. The nodes and edges are retrieved from the STRING tool and plotted using Cytoscape software. (e) The four cluster subnetworks were identified from the PPI network with the help of Cytoscape using the MCODE plug-in with a cluster score. (f) The expression of SERPINE2 in hepatoblastoma and normal liver in GSE131329. (g) The expression of SERPINE2 in hepatoblastoma and matched nontumor liver tissues in our hospital. (h) Immunohistochemistry detection of SERPINE2 expression in hepatoblastoma adjacent normal liver tissues. (Representative images were shown at ×40 (scale bar, 50 mm) via microscope.) (i) Immunohistochemistry detection of SERPINE2 expression in hepatoblastoma tissues. (Representative images were shown at ×40 (scale bar, 50 mm) via microscope.)

Article Snippet: Paraffin-embedded tissue slides were heated, dewaxed, and dehydrated, then repaired with H 2 O 2 and citric acid antigen, and sealed with goat serum, and polyclonal SERPINE2 rabbit primary antibody (1 : 50; GeneTex, USA) was incubated overnight (PBS was used instead of primary antibody as negative control); goat anti-rabbit secondary antibody was incubated the next day and incubated at room temperature for 60 min.

Techniques: Expressing, Software, Immunohistochemistry, Microscopy

Journal: Disease Markers

Article Title: Upregulation of SERPINE2 Results in Poor Prognosis of Hepatoblastoma via Promoting Invasion Abilities

doi: 10.1155/2022/2283541

Figure Lengend Snippet: Relationship between SERPINE2 expression and hepatoblastoma clinicopathological features.

Article Snippet: Paraffin-embedded tissue slides were heated, dewaxed, and dehydrated, then repaired with H 2 O 2 and citric acid antigen, and sealed with goat serum, and polyclonal SERPINE2 rabbit primary antibody (1 : 50; GeneTex, USA) was incubated overnight (PBS was used instead of primary antibody as negative control); goat anti-rabbit secondary antibody was incubated the next day and incubated at room temperature for 60 min.

Techniques: Expressing

Journal: Disease Markers

Article Title: Upregulation of SERPINE2 Results in Poor Prognosis of Hepatoblastoma via Promoting Invasion Abilities

doi: 10.1155/2022/2283541

Figure Lengend Snippet: SERPINE2 expression the hepatoblastoma cohort using the cutoff (low (IHC score < 5) vs. high (IHC score ≥ 5)) as a determinant of overall survival in the Kaplan-Meier analysis ( P < 0.001, log-rank test). IHC: immunohistochemistry.

Article Snippet: Paraffin-embedded tissue slides were heated, dewaxed, and dehydrated, then repaired with H 2 O 2 and citric acid antigen, and sealed with goat serum, and polyclonal SERPINE2 rabbit primary antibody (1 : 50; GeneTex, USA) was incubated overnight (PBS was used instead of primary antibody as negative control); goat anti-rabbit secondary antibody was incubated the next day and incubated at room temperature for 60 min.

Techniques: Expressing, Immunohistochemistry

Journal: Disease Markers

Article Title: Upregulation of SERPINE2 Results in Poor Prognosis of Hepatoblastoma via Promoting Invasion Abilities

doi: 10.1155/2022/2283541

Figure Lengend Snippet: Univariate and multivariate Cox proportional hazards analyses for the overall survival of hepatoblastoma.

Article Snippet: Paraffin-embedded tissue slides were heated, dewaxed, and dehydrated, then repaired with H 2 O 2 and citric acid antigen, and sealed with goat serum, and polyclonal SERPINE2 rabbit primary antibody (1 : 50; GeneTex, USA) was incubated overnight (PBS was used instead of primary antibody as negative control); goat anti-rabbit secondary antibody was incubated the next day and incubated at room temperature for 60 min.

Techniques:

Journal: Disease Markers

Article Title: Upregulation of SERPINE2 Results in Poor Prognosis of Hepatoblastoma via Promoting Invasion Abilities

doi: 10.1155/2022/2283541

Figure Lengend Snippet: SERPINE2 did not significantly affect HB proliferation but significantly inhibited early apoptosis in HepG2 and HUH6 cells. (a) EdU assays detect the proliferation ability of HepG2 cells in OE-SERPINE2 group and si-SERPINE2 group. (b) EdU assays detect the proliferation ability of HUH6 cells in OE-SERPINE2 group and si-SERPINE2 group. (c) Flow cytometry detected the apoptosis rate of HepG2 cells in OE-SERPINE2 group and si-SERPINE2 group. (d) Flow cytometry detected the apoptosis rate of HUH6 cells in OE-SERPINE2 group and si-SERPINE2 group.

Article Snippet: Paraffin-embedded tissue slides were heated, dewaxed, and dehydrated, then repaired with H 2 O 2 and citric acid antigen, and sealed with goat serum, and polyclonal SERPINE2 rabbit primary antibody (1 : 50; GeneTex, USA) was incubated overnight (PBS was used instead of primary antibody as negative control); goat anti-rabbit secondary antibody was incubated the next day and incubated at room temperature for 60 min.

Techniques: Flow Cytometry

Journal: Disease Markers

Article Title: Upregulation of SERPINE2 Results in Poor Prognosis of Hepatoblastoma via Promoting Invasion Abilities

doi: 10.1155/2022/2283541

Figure Lengend Snippet: Wound healing assay. (a, b) Wound healing assay detects the migration ability of HepG2 cells in OE-SERPINE2 group and si-SERPINE2 group. (c, d) Wound healing assay detects the migration ability of HUH6 cells in OE-SERPINE2 group and si-SERPINE2 group.

Article Snippet: Paraffin-embedded tissue slides were heated, dewaxed, and dehydrated, then repaired with H 2 O 2 and citric acid antigen, and sealed with goat serum, and polyclonal SERPINE2 rabbit primary antibody (1 : 50; GeneTex, USA) was incubated overnight (PBS was used instead of primary antibody as negative control); goat anti-rabbit secondary antibody was incubated the next day and incubated at room temperature for 60 min.

Techniques: Wound Healing Assay, Migration

Journal: Disease Markers

Article Title: Upregulation of SERPINE2 Results in Poor Prognosis of Hepatoblastoma via Promoting Invasion Abilities

doi: 10.1155/2022/2283541

Figure Lengend Snippet: The effects of SERPINE2 on epithelial-mesenchymal transition in HB cells. (a–d) Western blot analysis of E-cadherin and N-cadherin in HepG2 cells transfected with OE-SERPINE2 group and si-SERPINE2 group. (e–h) Western blot analysis of E-cadherin and N-cadherin in HUH6 cells transfected with OE-SERPINE2 group and si-SERPINE2 group.

Article Snippet: Paraffin-embedded tissue slides were heated, dewaxed, and dehydrated, then repaired with H 2 O 2 and citric acid antigen, and sealed with goat serum, and polyclonal SERPINE2 rabbit primary antibody (1 : 50; GeneTex, USA) was incubated overnight (PBS was used instead of primary antibody as negative control); goat anti-rabbit secondary antibody was incubated the next day and incubated at room temperature for 60 min.

Techniques: Western Blot, Transfection